RESUMO
Bacteroides fragilis is an anaerobic, Gram-negative, commensal bacterium of the human gut. It plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. Many recent studies have focused on the relationship between B. fragilis and human immunity, and indicate that B. fragilis has many useful probiotic effects. As inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether B. fragilis could inhibit pathogenic bacteria. Results showed that Vibrio parahaemolyticus was inhibited by B. fragilis in vitro, and that B. fragilis could protect both RAW 264.7 and LoVo cells from damage caused by V. parahaemolyticus. Using in vivo imaging, we constructed a light-emitting V. parahaemolyticus strain and showed that B. fragilis might shorten the colonization time and reduce the number of lux-expressing bacteria in a mouse model. These results provide useful information for developing B. fragilis into a probiotic product, and also indicate that this commensal bacterium might aid in the clinical treatment of gastroenteritis caused by V. parahaemolyticus.
Assuntos
Antibiose , Bacteroides fragilis/fisiologia , Diagnóstico por Imagem/métodos , Medições Luminescentes/métodos , Vibrioses/diagnóstico por imagem , Vibrioses/terapia , Vibrio parahaemolyticus/fisiologia , Animais , Bacteroides fragilis/crescimento & desenvolvimento , Linhagem Celular , Modelos Animais de Doenças , Doenças Transmitidas por Alimentos/terapia , Gastroenterite/terapia , Humanos , Intestinos/microbiologia , Camundongos , Probióticos , Células RAW 264.7 , Vibrio parahaemolyticus/crescimento & desenvolvimento , Vibrio parahaemolyticus/patogenicidadeAssuntos
Úlcera do Pé/microbiologia , Radiodermite/complicações , Vibrioses/etiologia , Vibrio alginolyticus/isolamento & purificação , Idoso , Doenças do Pé/etiologia , Úlcera do Pé/etiologia , Humanos , Masculino , Ossos do Metatarso/diagnóstico por imagem , Ossos do Metatarso/microbiologia , Osteomielite/diagnóstico por imagem , Osteomielite/etiologia , Água do Mar/microbiologia , Espanha , Vibrioses/diagnóstico por imagem , Microbiologia da ÁguaRESUMO
This report describes a case of a mycotic aneurysm of the infrarenal abdominal aorta infected by Vibrio mimicus, which is the only such case ever reported in the literature. The 80-year-old male patient was first treated for gastroenteritis for 7 days. Two months later, he was admitted to the hospital and the aneurysm was diagnosed by three-dimensional computed tomography. The aneurysm was excised and the restoration was done using a 16-mm Dacron straight graft. The cultivation of the excised aneurysm and the inflammatory fluid was positive for V. mimicus. The postoperative period was free of complications and the patient has remained asymptomatic during the 4-year follow-up.
